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Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

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Ying Chen

The SG-NEx project: a resource for long-read nanopore RNA sequencing in 5 human cancer cell lines

Team generated reference dataset for anyone to use, containing data for replicates of PCR-cDNA, direct cDNA, direct RNA, and short-read RNA across 5 cancer cell lines. Over 36 million reads per sample, available here: https://github.com/GoekeLab/sg-nex-data

YC: “the more complex the isoform expression, the more important long reads become” – isoform switching occurs frequently across cancer cell lines and needs full-length transcripts to identify.

Project has found over 8000 novel transcripts which are either novel isoforms or novel genes - download the data yourself to investigate.

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