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Rapid de novo assembly of the European eel genome from nanopore sequencing reads


Date: 20th January 2017 | Source: Nature Scientific Reports

Authors: Hans J. Jansen, Michael Liem, Suzanne A. Jong-Raadsen, Sylvie Dufour, Finn-Arne Weltzien, William Swinkels, Alex Koelewijn, Arjan P. Palstra, Bernd Pelster, Herman P. Spaink, Guido E. Van den Thillart, Ron P. Dirks, Christiaan V. Henkel.

We have sequenced the genome of the endangered European eel using the MinION by Oxford Nanopore, and assembled these data using a novel algorithm specifically designed for large eukaryotic genomes. For this 860 Mbp genome, the entire computational process takes two days on a single CPU. The resulting genome assembly significantly improves on a previous draft based on short reads only, both in terms of contiguity (N50 1.2 Mbp) and structural quality. This combination of affordable nanopore sequencing and light-weight assembly promises to make high-quality genomic resources accessible for many non-model plants and animals.

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