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Oxford Nanopore DNA and RNA Sequencing Devices

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Research products Research Real-time DNA and RNA sequencing — from portable to high-throughput devices. Q-Line products Q-Line Locked-down, research-validated devices for applied sequencing applications.
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Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

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Oxford Nanopore Technologies Fully scalable, real-time DNA/RNA sequencing technology Oxford Nanopore Diagnostics

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Flongle

Flongle

Adapter to enable small, rapid nanopore sequencing tests, for mobile or desktop sequencers
MinION Mk1B

MinION Mk1B

Your personal nanopore sequencer, putting you in control
MinION Mk1C

MinION Mk1C

Your personal nanopore sequencer including compute and screen, putting you in control
GridION Mk1

GridION Mk1

Higher-throughput, on demand nanopore sequencing at the desktop, for you or as a service
PromethION 2 Solo

PromethION 2 Solo

Adapt existing hardware for high-depth nanopore sequencing, for you or as a service. Pre-order only
PromethION 2

PromethION 2

A fully integrated device for high-depth nanopore sequencing with minimal cost outlay, for you or as a service. Pre-order only
PromethION 24/48

PromethION 24/48 A100

Ultra-high throughput, on-demand nanopore sequencing, for you or as a service
 

Product comparison

Overview

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 2 Solo PromethION 2 PromethION 24 PromethION 48
Read length Nanopores read the length of DNA presented to them. Longest read so far: > 4 Mb.
Number of flow cells per device 1 1 1 5 2 2 24 48
Theoretical maximum output per device, Kit 10/11 chemistry 2.8 Gb 50 Gb 50 Gb 250 Gb Up to 580 Gb Up to 580 Gb Up to 7 Tb Up to 14 Tb
Theoretical maximum output per flow cell, Kit 10/11 chemistry 2.8 Gb 50 Gb 50 Gb 50 Gb Up to 290 Gb Up to 290 Gb Up to 290 Gb Up to 290 Gb
Theoretical maximum output per device, Kit 12 chemistry Up to 1.6 Gb Up to 30 Gb Up to 30 Gb Up to 150 Gb Up to 340 Gb Up to 340 Gb Up to 4 Tb Up to 8 Tb
Theoretical maximum output per flow cell, Kit 12 chemistry Up to 1.6 Gb Up to 30 Gb Up to 30 Gb Up to 30 Gb Up to 170 Gb Up to 170 Gb Up to 170 Gb Up to 170 Gb
Theoretical max output when system is run for 16 hours at 420 bases / second (kit 10/11 chemistry) or 250 bases / second (kit 12 chemistry). Outputs may vary according to library type, run conditions, etc. * Theoretical max output when system is run for 72 hours at 420 bases / second (kit !0/11 chemistry) or 250 bases / second (kit 12 chemistry). Outputs may vary according to library type, run conditions, etc.
System access From $1,460 From $1,000 From $4,900 From $49,995 From $10,455 From $59,995 From $225,000 From $310,000
Availability Buy now Buy now Buy now Buy now Register to pre-order Register to pre-order Buy now Buy now
 

Purchasing information

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 2 Solo PromethION 2 PromethION 24 PromethION 48
System Price Starter Pack $1,460 $1,000 $4,900 $49,995 $10,455 $59,995 $225,000 $310,000
Number of flow cells included 12 1 6 60 8 48 192 288
Number of Library preparation kits included - 1 1 10 2 8 42 48
Software Licence and warranty included - - 12 months 12 months 12 months 12 months 12 months 12 months
System Price CapEx - - $9,300 $69,955 $15,445 $79,995 $325,000 $450,000
Software Licence and warranty included in CapEx - - 12 months 12 months 12 months 12 months 12 months 12 months
Software Licence and Warranty annual charge - - $300 $12,500 $1,000 $12,500 $35,000 $45,000
 

Output and price per Gb

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 2 Solo PromethION 2 PromethION 24 PromethION 48
Number of channels per flow cell 126 512 512 512 2675 2675 2675 2675
Number of flow cells per device 1 1 1 5 2 2 24 48
Price per flow cell $90 $900 - $475 $900 - $475 $900 - $475 $2,000 - $625 $2,000 - $625 $2,000 - $625 $2,000 - $625
Run time 1 min - 16 hours 1 min - 72 hours 1 min - 72 hours 1 min - 72 hours 1 min - 72 hours 1 min - 72 hours 1 min - 72 hours 1 min - 72 hours
Yields in field are dependent on sample and preparation methods. Users can get outputs in the following ranges per flow cell when utilising the latest chemistries and protocols 1 - 2 Gb 10 - 30 - 50 Gb 10 - 30 - 50 Gb 10 - 30 - 50 Gb 100 - 200 - 300 Gb 100 - 200 - 300 Gb 100 - 200 - 300 Gb 100 - 200 - 300 Gb
Price per Gb for different data yields per flow cell. Yields vary according to sequencing kit used and sample and preparation methods.
† yield value relevant for Kit 12 sequencing chemistry		 @1† - 2 Gb
$90 per flow cell:
$90 - 45		 @10† - 30 - 50 Gb
$900 per flow cell:
$90 - 30 - 18
$790 per flow cell:
$79 - 26 - 16
$675 per flow cell:
$68 - 23 - 14
$500 per flow cell:
$50 - 17 - 10
$475 per flow cell:
$48 - 16 - 9.5		 @10† - 30 - 50 Gb
$900 per flow cell:
$90 - 30 - 18
$790 per flow cell:
$79 - 26 - 16
$675 per flow cell:
$68 - 23 - 14
$500 per flow cell:
$50 - 17 - 10
$475 per flow cell:
$48 - 16 - 9.5		 @10† - 30 - 50 Gb
$900 per flow cell:
$90 - 30 - 18
$790 per flow cell:
$79 - 26 - 16
$675 per flow cell:
$68 - 23 - 14
$500 per flow cell:
$50 - 17 - 10
$475 per flow cell:
$48 - 16 - 9.5		 @100† - 200 - 300 Gb
$1,600 per flow cell:
$16 - 8 - 5
$1,120 per flow cell:
$11 - 6 - 4
$940 per flow cell:
$9 - 5 - 3.1
$680 per flow cell:
$7 - 3.4 - 2.3
$625 per flow cell:
$6 - 3 - 2		 @100† - 200 - 300 Gb
$1,600 per flow cell:
$16 - 8 - 5
$1,120 per flow cell:
$11 - 6 - 4
$940 per flow cell:
$9 - 5 - 3.1
$680 per flow cell:
$7 - 3.4 - 2.3
$625 per flow cell:
$6 - 3 - 2		 @100† - 200 - 300 Gb
$1,600 per flow cell:
$16 - 8 - 5
$1,120 per flow cell:
$11 - 6 - 4
$940 per flow cell:
$9 - 5 - 3.1
$680 per flow cell:
$7 - 3.4 - 2.3
$625 per flow cell:
$6 - 3 - 2		 @100† - 200 - 300 Gb
$1,600 per flow cell:
$16 - 8 - 5
$1,120 per flow cell:
$11 - 6 - 4
$940 per flow cell:
$9 - 5 - 3.1
$680 per flow cell:
$7 - 3.4 - 2.3
$625 per flow cell:
$6 - 3 - 2
 

Sample Preparation

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 2 Solo PromethION 2 PromethION 24 PromethION 48
Starting Material DNA, amplicons, cDNA, Direct RNA
Sample input per library Protocols from 10 pg - 1 ug
Sample Preparation time Rapid Kit: 10 minutes, Ligation Kit: 60 minutes, other protocols and timings also available
Library Preparation cost per run $25 - $50 $99 $99 $99 $99 $99 $99 $99
Multiplex options Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96

PCR Barcodes 1 - 96		 Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible		 Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible		 Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible		 Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible		 Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible		 Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible		 Rapid Barcodes: 1 - 12
Native Barcodes: 1 - 96
PCR Barcodes 1 - 96
Combinatorial Barcodes > 2K combinations possible
 

Specifications

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 2 Solo PromethION 2 PromethION 24 PromethION 48
Power requirement Powered by MinION / GridION Powered by Laptop / MinIT 25 W 800 W Specification to be determined.
Please see the P2 Solo store page for details		 Specification to be determined.
Please see the P2 store page for details		 2.2 kW 2.2 kW
Dimensions W 105, H 23, D 8 mm W 105, H 23, D 33 mm W 140, H 30, D 116 mm W 365, H 220, D 360 mm Sequencer: W 590 H 190 D 430 mm;
Data Acquisition unit W 178 H 440 D 470 mm		 Sequencer: W 590 H 190 D 430 mm;
Data Acquisition unit W 178 H 440 D 470 mm
Weight 20 g 87 g 450 g 11 Kg Sequencer: 28 Kg
Data Acquisition unit: 25 Kg		 Sequencer: 28 Kg
Data Acquisition unit: 25 Kg
Data Transfer - - Ethernet/MicroSD/USB/GSM/Wifi 1 Gb Ethernet Dual 10 Gb Fibre or Ethernet Dual 10 Gb Fibre or Ethernet
Storage - - 1 TB SSD 4 TB SSD 60 TB SSD 60 TB SSD
RAM - - 8 GB 64 GB 512 GB 512 GB
 

Platform

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 2 Solo PromethION 2 PromethION 24 PromethION 48
Read length Nanopores read the entire length of the fragment of DNA/RNA presented to them. Longest read so far: > 4Mb.  Accuracy is maintained throughout the fragment.
Single Molecule Accuracy Compatible with Kit 10/11 Chemistry for single molecule accuracy >98%. Compatible with Kit 10/11 chemistry for single molecule accuracy >98%. Compatible with Kit 12 chemistry for single molecule accuracy >99%, duplex enabled. Please see our accuracy page for a full explanation of platform performance
Consensus Accuracy Please see our accuracy page for a full explanation of platform performance
Sample Preparation time Rapid Kit: 10 minutes, Ligation Kit: 60 minutes, other protocols and timings also available
Modified base detection Yes - Base modification information available in raw signal
Time to 1st usable data 2 minutes
Analysis tools Community developed, Tutorials, EPI2ME. More information
                 
 

Application

  Flongle MinION Mk1B MinION Mk1C GridION Mk1 PromethION 2 Solo PromethION 2 PromethION 24 PromethION 48
Large whole genome Sequencing Library prep QC - Yes Yes Yes Yes Yes Yes
Small whole genome Sequencing Yes Yes: Low to medium plex Yes: Low to medium plex Yes: Low to medium plex Yes: Highly multiplexed Yes: Highly multiplexed Yes: Highly multiplexed Yes: Highly multiplexed
Targeted Sequencing Yes: Low to medium plex, and adaptive sampling Yes: Medium to high plex, and adaptive sampling Yes: Low to medium plex, and adaptive sampling Yes: Low to medium plex, and adaptive sampling Yes: Highly multiplexed Yes: Highly multiplexed Yes: Highly multiplexed Yes: Highly multiplexed
Metagenomics Yes: Species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID Yes: Quantitative species ID
RNA Sequencing Yes: Isoform Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment Yes: Isoform & expression from same experiment
Epigenetics Yes Yes Yes Yes Yes Yes Yes Yes
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