Welcome to the Nanopore Community

Connecting nanopore sequencers from around the world

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What is the Nanopore Community?

Our community helps to bring everyone using nanopore technology together. New users get started quicker with access to Nanopore Learning. Discussion and collaboration drives experimental success with full access to documentation and support and as a member of our community you will help shape the next generation of our products and software.

Learn

Online courses support your future experiments from sample to result.

Plan

Identify and discuss the most appropriate extraction method, sequencing protocol and analysis workflow to use for your experiment.

Run

Download the latest software and protocol updates. Learn about the latest techniques and applications updates.

Experiment planning

Protocol Library

A comprehensive database containing over 86 Oxford Nanopore Technologies protocols for step-by-step experimental guidance. Whole genome sequencing and metagenomics to cDNA and RNA sequencing are covered.

Protocol Builder

End-to-end experimental planning using our interactive protocol builder. Define your aim, then extraction process, library preparation, and experimental analysis, the protocol builder simplifies the experimental process, and provides a complete tailored workflow.

Protocol Selector

Detailed guidance and specific recommendations for library preparation based upon your sample type, input quantity and experimental priorities.

Online training with Nanopore Learning

MinION Mk1C and Mk1B

Getting started

Help with planning and step-by-step walkthroughs of your first MinION experiment

Technology introduction

Prepare to use nanopore sequencing technology with our 7-part video training course

Support & documentation

Help and technical documentation

Discussions

Posts and discussions about every aspect of using nanopore technology with our global audience

Extraction methods

Extract DNA from a range of different biological samples, including plants, animal tissue, cell lines, soil, FFPE samples, and bacteria. Detailed information sheets and specific recommendations are provided for various tissue types.

In addition to extraction protocols, we have prepared a collection of useful tips and tricks, including advice on RNA QC, the effects of contaminants, and cell storage, to help you get the best results from your nanopore sequencing experiment.

Bioinformatics resources

Our bioinformatics offerings include research tools, our EPI2ME Labs and EPI2ME products. Our research tools are available through our GitHub and are suited for users with bioinformatics experience. A collection of tutorials to introduce best practise solutions to research questions is available through EPI2ME Labs. These tutorials include step-by-step instructions and are packaged with example datasets. Topics include:

  • File formats that will be encountered during Nanopore sequence analysis (e.g. FAST5, FASTQ, BAM and VCF)
  • Review of sequence collections to collate and present summary statistics for QC purposes
  • An introduction to genome assembly and consensus sequence polishing
  • Analysis of SARS-CoV-2 genome sequence data prepared using ARTIC-based protocols
  • Transcriptome analysis for differential gene expression and for the identification and characterisation of isoforms
  • Detection of genetic variants — from SNPs in haploid genomes through to SV in human genomes

These tutorials are provided through a Jupyter Labs server running in a docker container. Installation instructions are available for Linux, Windows and macOS.