- MinION的小型适配器，适合更小型的测试或实验
- 单次使用、按需、成本优廉的测序
- 适用于质控检查、扩增子、更小型的基因组、靶向区域，或者对诊断/其它测试感兴趣的研究者
- 可用MinIT 支持IT/软件需求
如有下列需求，请选用Flongle
- 希望分析更小型的样本
- 更倾向于运行单个样本，而不是混样测序/加条码标签
- 在开展更大型的实验之前，希望对一个样品实施快速的质控检查
如有下列需求，请选用 MinION：
- 希望测序成本在1000 美元
- 希望无需等待的即时测序
- 希望能在实验室外进行实地测序
- 希望达到每48 小时10-20 Gb数据量
- 避免资本性支出的投入。
- 包含多个测序装置，一个计算模块
- 一次使用多达 5 个 MinION 测序芯片
- 台式处理器完成对大数据量的实时处理
- 高效、实时的程序应用，例如 Read Until……
如有下列需求，请选用 GridION X5：
- 希望将纳米孔测序作为一项服务提供
- 投资方式可从资本性支出和耗材支出中进行选择
- 希望从事较大的测序项目（每 48 小时 50-100Gb 数据量）
- 希望直接在测序仪上进行碱基序列读取——无需辅助设施。
- 高通量、高样本数的台式系统
- 模块化设计：多达 48 个测序芯片，各有多达 3,000 个纳米孔通道（总计达 144,000 个）
- 测序芯片既可单独也可同时运行
- 大容量测序，但工作流程与 MinION相同
如有下列需求，请选用 PromethION：
- 希望将纳米孔测序作为一项服务提供
- 对具有庞大数据量的项目 (Tb) 感兴趣
- 希望能对大量样本进行按需测序服务
- 避免资本性支出的投入。
如有下列需求，请选择纳米孔测序：
- 超长读长
- 实时数据读取，实现实时数据分析
- 直接对所感兴趣的分子而非替代标记进行分析
- 快速、简捷的文库制备（最短只需 5 分钟）
- 灵活的工作流程，包括多重测序和自动操作
- 可扩展的测序解决方案，定价适合于任何经费情况，且不仅局限于实验室内使用。
Product specifications comparison
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| Mk 1 MinION Commercially available |
GridION X5 (5x MinIONs + Compute) |
Single PromethION Flow Cell | PromethION (48 Flow Cells) | |
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| Availability | Start using MinION | Contact to order now | Register for PEAP | Register for PEAP |
| Number of channels available for sequencing | Up to 512 | up to 2,560 | Up to 3,000 | Up to 144,000 |
| Your Sample | ||||
| Sample input requirement PCR free1 | 10 pg - 1 µg | 10 pg - 1 µg | 10 pg - 1 µg | 10 pg - 1 µg |
| Flow cell input volume2 | 75 µl | 75 µl | 35 µl per sample well (4 wells in a flow cell) | 35 µl per sample well (192 wells in a PromethION) |
| Sample preparation time 1D | 10 minutes | 10 minutes | 10 minutes | 10 minutes |
| System Operation | ||||
| Run time2 | 1 minute - 48 hours | 1 minute - 48 hours | 1 minute - 48 hours | 1 minute - 48 hours |
| Flow cell lifetime3 | ~72 hrs | ~72 hrs | ≥ 72hrs | ≥ 72hrs |
| Time to first usable read (data available in real time) | 2 minutes | 2 minutes | 2 minutes | 2 minutes |
| Number of reads at 10 kb at standard speed (250 bps)4 | Up to 4.4 M | Up to 4.4 M | Up to 26 M | Up to 1,250 M |
| Read length | The longest reported read by a MinION user is now approaching 1Mb | The longest reported read by a MinION user is now approaching 1Mb | The longest reported read by a MinION user is now approaching 1Mb | The longest reported read by a MinION user is now approaching 1Mb |
| 1D Yield4 at 450 bps in 48 hours | Up to 40 Gb (Theoretical maximum) | Up to 40 Gb (Theoretical maximum) | Up to 233 Gb (Theoretical maximum) | Up to 11 Tb (Theoretical maximum) |
| 1D Yield5 at 450 bps (October 2016 release) | 5-10 Gb | N/A | N/A | N/A |
| 1D Yield5 at 450 bps (MinKNOW 1.4 Feb 2017 release) | 10-20 Gb | 50-100 Gb | N/A | N/A |
| Base calling accuracy6 | Up to 99% | Up to 99% | Up to 99% | Up to 99% |
| Raw data available | Yes | Yes | Coming soon | Coming soon |
| Modified base detection | Yes | Yes | Yes | Yes |
| Data analysis | Local offline/online | Local offline/online | On Unit / Local offline | On Unit / Local offline |
| System Specifications | ||||
| Power requirement | USB 3 (1 watt) | 600 W | ~2 kW | ~2 kW |
| Potential raw data requirements (Bytes per second) | Up to 5 MBps | Up to 25 MBps | Up to 30 MBps | Up to 1.44 GBps |
| Dimensions | W 105, H 23, D 33 mm | W 360 H 200 D 360 mm | W 440, H 400, D 240 mm | W 440, H 400, D 240 mm |
| Weight | 87 g | 10 Kg | 40 Kg | 40 Kg |
| Facility modifications required | None | None | None | None |
| Price on Application | ||||
| Annual service contract7 | None | $15k Support | $15k (compute module support) | $15k (compute module support) |
| Reagent cost per run8 | $ 99 | $99 | POA | POA |
| Flow cell cost (depending on order type and volume)8 | $500 - $900 | $299-900 | POA | POA |
| Instrument access fee | Starter kit (includes MinION and all materials for 2 runs) $1000 | No instrument cost | No instrument cost | No instrument cost |
- Higher sample input will result in a higher yield. The system can, in principle, detect just one molecule subject to a long enough wait and random diffusion.
- Data is available within a minute of the run starting. If a user reaches the answer necessary quickly they can simply stop the run, wash, load and analyse another sample. Current scripts are set to run for 48 hours but these can be stopped and started as necessary.
- Current default run time is 48 hours although it is possible to run the system for longer if a user chooses to. Longer default run conditions are in development along with other design features that can extend a flow cell life. The system can run at different speeds depending on user requirements. This is measured in bases that pass through the pore per second (bps). Current default speed on the MinION is 450 bps. Speeds may increase in future beyond those shown.
- Maximal numbers are shown here. Actual yields may vary according to sample type, sample preparation methods, fragmentation methods, user chosen instrument settings and other factors.
- Yields may vary according to sample type, sample preparation methods, fragmentation methods, user chosen instrument settings and other factors.
- Accuracy may depend on base calling software chosen, user chosen run settings and other factors. Future versions of the system may exceed figures shown here.
- Upgrades or replacements will be made available promptly. Customers may propose alternative service arrangements and bespoke contracts.
- POA: Price on application. Detailed pricing breakdown for PromethION consumables are not yet available. Price per Gb will be competitive with other market leading sequencing systems.
