Products
Applications

Applications

Nanopore sequencing offers advantages in all areas of research. Our offering includes DNA sequencing, as well as RNA and gene expression analysis and future technology for analysing proteins.

Learn about applications
View all Applications
Resources Investors Careers News About Store Community Contact

Sequencing short fragments with nanopore technology​

Nanopore technology enables the sequencing of short to ultra long DNA or RNA fragments, providing a universal sequencing platform capable of observing the full scope of biology

 

More applications

Product range

Figure 1: Choose your read length cut off using the Minimum Read Length filter within MinKNOW

Fragment ladder

Figure 2. A DNA ladder is run with 2 different minimum read length settings: A) 200 bp and B) 20 bp. With a setting of 20 bp, fragments below 200 bases in length are recorded for further analysis.

How it works

​SFM is enabled by a new configuration setting in MinKNOW (first released to the Nanopore Community in March 2022 - see Figure 1). During run setup a user simply selects their preferred minimum read length. 

The setting is used to direct the software to write sequencing files to disk from the minimum size selected. The performance of this is demonstrated in Figure 2, with the experimental outputs of running a DNA ladder with the minimum length set to A) 200 bp and B) 20 bp shown — the read length distribution matches the chosen software setting.

​Applications​

We envision that SFM will enable the community to deploy nanopore sequencing for use in exciting areas such as cell-free DNA analysis, sequencing of FFPE samples, or liquid biopsy research. Users will be able to pair SFM with other nanopore capabilities such as highly scalable outputs, amplification-bias free workflows and the detection of methylation without additional preparation.​

We'd love to hear your ideas, so please follow the link below to leave us feedback on the applications you would like to pursue with SFM.

Clinical research samples

Give us your feedback

We always want to have our customer's input at the heart of what we do and the products we develop. We're incredibly excited about the introduction of SFM, and we'd love to hear your ideas on where this new feature can be applied.

Please click the button below to submit your thoughts, ideas and improvements for SFM.

Flongle

Flongle

Adapting MinION and GridION for smaller, routine tests and analyses. Low plex targeted sequencing, RNA isoform analysis, and quality control applications.

View Flongle
MinION Mk1B

MinION Mk1B

Access the benefits of nanopore technology from just $1,000 — suitable for targeted sequencing and gene expression studies.

View MinION
MinION Mk1C

MinION Mk1C

Integrated sequencing and analysis in a powerful handheld device — suitable for targeted sequencing and gene expression studies.

View MinION
GridION

GridION

From genome assembly to gene expression, run multiple experiments on-demand using 5 independent MinION flow cells.

View GridION
PromethION

PromethION

Flexible, population-scale sequencing using up to 48 independent, high-capacity flow cells — complete genomic and transcriptomic characterisation of large sample numbers.

View PromethION
VolTRAX

VolTRAX

Automated sample extraction and library preparation.

View VolTRAX
Open a chat to talk to our sales team